Once you reconstitute a peptide, you need to know its concentration to work accurately. The maths is simple.
The core formula
Concentration (mg/mL) = peptide mass (mg) ÷ water volume (mL). A 10 mg vial reconstituted with 2 mL gives 5 mg/mL.
Choosing your volume
More water lowers the concentration (gentler increments per unit volume); less water raises it. Pick a volume that makes your intended measurements convenient on the syringe scale.
From mg/mL to a draw
To withdraw a target mass, divide it by the concentration: 0.25 mg from a 5 mg/mL solution = 0.05 mL. Many researchers use our on-site peptide calculator to avoid arithmetic slips.
For research use only. Not for human or veterinary use, consumption, or diagnostic application.



